Your end supports for the gluelam beam will need to be capable of supporting the same bearing load as half of the 2x4's that were in the wall. (1/2 on each end)
If your wall is 9 feet long, you should have roughly 8 2x4's in the wall. 4 2x4's have a net cross section of:
1.5 x 3.5 (net dimensions of finished lumber) = 5.25 square inches.
4 x 5.25 = 21 square inches of bearing.
a single 4x4 is only going to have about 10.5 square inches. However, if you combine a couple of studs with a simpson bracket tied into adjoining studs in the adjacent walls, you might be able to get there. This all depends on what you are supporting above it.
The other major issue is the difference between a spread load (the bearing wall sitting on a plate, whose load is spread along the entire length of the plate) and converting that to two single point loads. What are these point loads going to sit on? My guess is you would have to reinforce whatever is beneath these posts so that you don't punch through the floor or cause a footing in your crawlspace to fail.
Ideally, you should hire a structural engineer to come out and do a load trace and recommend member sizes to you. That way you do it right.
Please don't rip out your sawzall and start cutting until you have paid a professional to tell you how to do this.